Award details

Flowering time control in sugar beet

Reference	BBS/E/C/00005996
Principal Investigator / Supervisor	Dr Euphemia Mutasa-Gottgens
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	221,831
Status	Completed
Type	Institute Project
Start date	01/04/2008
End date	31/03/2012
Duration	48 months

Abstract

The proposed project has two main objectives: 1. To identify, isolate, and sequence sugar beet genetic elements orthologous to Arabidopsis genes known to integrate the vernalization and photoperiod dependant pathways to flowering. 2. To determine the expression profiles of the isolated genes under inductive and non-inductive growth conditions in biennial and annual sugar beet genotypes. Experimental approach Objective 1: Sugar beet orthologues of Arabidopsis genes will be sought using standard cloning techniques based on PCR, genome walking and genomic library screening. The genomic structure and sequence of the genes in biennial beet will be determined and compared with known sequences from other species. Genes that integrate vernalization and photoperiodic pathways will be isolated in the first instance and as possible; their function verified by complementation of Arabidopsis mutants and confirmed in transgenic sugar beet. Objective 2: Results from objective 1 are expected to provide gene candidates for manipulation to regulate flowering time in sugar beet. However, appropriate genetic manipulation strategies will depend on knowledge of how the genes respond to vernalization and photoperiod. This will be determined by measuring their expression in vernalized sugar beet plants under both inductive long-day and non-inductive short day photoperiods.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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