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A new generation of insect resistant GM crops: Transgenic wheat synthesising the aphid alarm signal

Reference	BBS/E/C/00005010
Principal Investigator / Supervisor	Professor John Pickett
Co-Investigators / Co-Supervisors	Professor Toby Bruce
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	153,247
Status	Completed
Type	Institute Project
Start date	01/12/2008
End date	30/11/2013
Duration	60 months

Abstract

The alarm pheromone for many aphids is the sesquiterpene (E)-beta-farnesene (EBf). This project aims to transform wheat plants with an EBf synthase from Mentha x piperita and examine behavioural responses of aphids and their natural enemies to these plants. The main objective is to develop transgenic wheat lines that repel aphids by emission of their alarm pheromone, evaluating this at both lab and field scale. Specific objectives are: 1) Transformation of wheat plants with EBf synthase gene 2) Molecular and phenotypic characterisation of transformed plants 3) Aphid alarm response bioassay to the transformed plants 4) Aphid olfactometer repellency bioassay: 5) Aphid settling test in field simulator 6) Parasitoid attraction bioassay 7) Parasitoid foraging bioassay 8) Field trials: Two field experiments will be conducted to determine aphid infestation levels of the transformed plants under typical field conditions.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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