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Analyses of aphid sodium channels and prediction of pesticide interactions

Reference	BBS/E/C/00004919
Principal Investigator / Supervisor	Professor Linda Field
Co-Investigators / Co-Supervisors	Dr Thomas Davies
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	89,347
Status	Completed
Type	Institute Project
Start date	16/04/2008
End date	15/04/2011
Duration	36 months

Abstract

1) To annotate the full length gene encoding the sequence of the A. pisum sodium channel from the genome sequence, identify the possible splice variants for the final protein product and clone the full length cDNA from A. pisum and/or Myzus persicae (either as one or several clones). 2) To produce a homology model of the Myzus persicae sodium channel and use this to identify the locations of mutations known to affect binding of pyrethroids to the sodium channel of aphids (it is highly likely that there will be very strong similarity between the channels in different aphid species) and hence locate critical amino acids involved in binding at this site. 3) To produce further models for regions of the aphid channel equivalent to the binding sites of ligands of mammalian channels and thus identify critical amino acids likely to be involved in binding of this type of ligand (especially regions known to be involved in binding of both insecticides and local anaesthetics). 4) To over-express aphid sodium channels in the yeast Pichia pastoris system for future compound validation. 5) To use the information obtained to design new hyperactive insecticidal molecules and those likely to overcome resistance and/or be more selective for insect channels (the synthesis of such molecules would be beyond the scope of the current project).

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	Crop Science, Plant Science, Structural Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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