Award details

Development of the lightscanner as a generic platform for novel allele discovery

Reference	BBS/E/C/00004882
Principal Investigator / Supervisor	Dr Andrew Phillips
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	28,736
Status	Completed
Type	Institute Project
Start date	01/09/2007
End date	31/08/2008
Duration	12 months

Abstract

The TILLING protocol has been established at Rothamsted Research to identify novel alleles in candidate genes associated with important traits in wheat. The current protocol uses mismatch cleavage of genomic PCR ( Polymerase Chain Reaction) products followed by denaturing polyacrylamide gel electrophoresis. While this is very sensitive and capable of high throughput, the method is expensive, laborious and technically demanding. We recently conceived an alternative method of mutation discovery using high-resolution melt curve analysis to identify point mutations in heteroduplex DNA. We propose to establish this new method on the Lightscanner platform in order to assess the feasibility, utility and throughput of the technique for high throughput mutation detection in wheat. We anticipate that the technique will be transferable to other species and will be particularly useful in diploid organisms where low mutation rates demand high rates of throughput.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	Crop Science, Plant Science, Technology and Methods Development
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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