Award details

BBSRC CASE studentship: Understanding resistance to light leaf spot to limit resistance breakdown and sustain oilseed rape gross margins

Reference	BBS/E/C/00004844
Principal Investigator / Supervisor	Professor Bruce Fitt
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	2,936
Status	Completed
Type	Institute Project
Start date	01/10/2006
End date	30/09/2009
Duration	36 months

Abstract

This project will use a mapping population of doubled haploid (DH) lines (produced by KWS UK Ltd.) segregating for resistance to Pyrenopeziza brassicae. The resistance gene(s) present in the mapping population will be mapped and studied. Microsatellite markers will be used to genetically map the doubled haploid population and plants will be grown and inoculated under controlled environment conditions to assess their disease resistance phenotype. These data, together with an improved knowledge of synteny between Arabidopsis thaliana and Brassica napus genomes, will be used to map the resistance locus/loci and identify candidate resistance genes. New techniques have been developed at Rothamsted to investigate host-pathogen interactions and to discriminate between different types of resistance operating in different lines and tissues of oilseed rape. These techniques include quantitative PCR (qPCR), which enables the amount of pathogen DNA in tissue to be quantified and its rate of growth and location to be assessed and genetic transformation of fungal isolates with reporter genes such as GFP to enable the pathogen to be seen by fluorescence in live leaf tissue (Eckert et al., 2005). These new techniques and new KWS UK Ltd. lines will be used to understand the resistance of oilseed rape to P. brassicae and the symptomless growth of the pathogen. During the project, P. brassicae isolates may be transformed with fluorescent reporter genes and used to visualise the effects of resistance on in planta growth.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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