Award details

BBSRC CASE studentship: Interactions between canker pathogens in oilseed rape examined using molecular reporters

Reference	BBS/E/C/00004761
Principal Investigator / Supervisor	Professor Bruce Fitt
Co-Investigators / Co-Supervisors	Dr Neal Evans
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	7,233
Status	Completed
Type	Institute Project
Start date	03/10/2005
End date	02/10/2008
Duration	36 months

Abstract

Objectives: 1. To investigate interactions between Leptosphaeria maculans and Leptosphaeria biglobosa on oilseed rape (Brassica napus) and the effects of triazole fungicides on these interactions and pathogen development. 2. To investigate the difference in susceptibility to triazoles between L. maculans and L. biglobosa. In in vitro studies, L. biglobosa was shown to have lower susceptibility than L. maculans, but the trend was reversed in field and controlled environment experiments with the cultivar Apex. Experiments will be done with a number of cultivars to determine whether the increased sensitivity of L. biglobosa in plants was due to host-pathogen incompatibility with the cultivar Apex or another factor. The transport of triazoles in plants will also be examined using gas chromatography techniques.3. To explore the genetic mechanisms for differences in triazole sensitivity between the Leptosphaeria species. The previously unsequenced Erg11 gene of L. biglobosa will be sequenced and compared with L. maculans Erg11. 4. To investigate growth of L. maculans and L. biglobosa between the leaf lesion and the stem in a series of controlled environment experiments and using samples taken from field experiments. Quantitative PCR will be used to measure the quantities of DNA in sections of petiole.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search