Award details

Exploitation of natural plant biodiversity for the presticide-free production of food (BIOEXPLOIT)

Reference	BBS/E/C/00004755
Principal Investigator / Supervisor	Professor Kim Hammond-Kosack
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	44,146
Status	Completed
Type	Institute Project
Start date	01/10/2005
End date	30/09/2010
Duration	60 months

Abstract

The overall objective of this sub-project is to improve the diversity of race-non-specific resistance mechanisms available in hexaploid wheat to combat Fusarium ear blight (FEB) disease. Seven specific objectives will be pursued: 1. To explore the cell biology of resistance mechanisms to FEB in newly identified resistance wheat accessions. This will use the already available Fusarium GUS and GFP reporter strains. This study will determine the exact point of hyphal arrest. 2. To quantify Fusarium biomass in infected grain and relate this to the level and profile of deoxynivalenol (DON) mycotoxins. 3. To identify which resistance genotypes confer a DON-mediated resistance phenotype. This will be achieved by parallel inoculations with DON+ and DON- strains. 4. To determine whether any of the novel resistance sources selected for this project contain any of the previously identified QTLs for resistance. This will involve SSR marker analysis. 5. To use a range of promoter TRI:GUS or promoter TRI:GFP transgenic reporter Fusarium strains to determine the onset of DON mycotoxin production in the tissues of novel resistance sources. 6. to explore resistance mechanism synergy. This will be achieved by examining the reactions of F1 plants generated by sexually crossing two different resistance genotypes that appear to operate via distinct mechanisms / have different QTLs /or no known QTLs 7. to determine the field reaction under UK conditions of a new set of resistant Chinese germplasm (non-Sumai-3 based). Includes studentship.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	Crop Science, Microbial Food Safety, Microbiology, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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