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The relationship between patterns of gluten protein synthesis and deposition and grain processing quality: a transgenic approach

Reference	BBS/E/C/00004716
Principal Investigator / Supervisor	Professor Peter Shewry
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	251,799
Status	Completed
Type	Institute Project
Start date	01/06/2005
End date	13/05/2010
Duration	59 months

Abstract

It is well established that the gluten proteins of wheat are the major determinants of the visco-elastic properties that allow doughs to be processed into bread, pasta and many other food products. Although it is usual to mill the whole starchy endosperm of the grain to produce white flour, this tissue is not homogenous, with evidence for gradients in protein amount and type between the sub-aleurone and peripheral cell layers and the central starchy endosperm cells. Furthermore, there is evidence that two pathways of protein deposition operate, although there is little known about how the operation of these relate to different proteins and cell types. We consider that it is crucial to understand the precise patterns of gluten protein synthesis and deposition as these will determine the structures, composition and properties of the glutenin polymers and hence the end use quality. The objectives of the research are therefore: 1. to determine the distribution of the major types of gluten protein within the endosperm of wheat and their presence in vacuolar and/or ER-derived protein bodies in the different cell types, using monoclonal antibodies, epitope tagging and grain fractionation. 2. to manipulate the gluten protein compositions of the sub-aleurone and central parts of the starchy endosperm by using endogenous HMW and LMW subunit promoters to drive transgene expression. 3. to determine the relationship between the composition and deposition pathway of gluten proteins and grain functional properties by analysis of flour fractions derived from the sub-aleurone and starchy endosperm of control and transgenic lines.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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