Award details

Isolation and characterization of sugar beet genes involved in floral induction

Reference	BBS/E/C/00004668
Principal Investigator / Supervisor	Dr Euphemia Mutasa-Gottgens
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	298,159
Status	Completed
Type	Institute Project
Start date	01/04/2004
End date	31/03/2008
Duration	48 months

Abstract

The proposed project has two main objectives: 1.To identify, isolate, and sequence sugar beet genetic elements that are homologs/orthologs of key Arabidopsis genes known to integrate the vernalization and photoperiod dependant pathways to flowering. 2.To determine the expression profiles of the isolated genes during flowering in biennial sugar beet, annual sugar beet, and annual wild Beta spp. Experimental approach - Objective 1: Sugar beet ortho/homologs of Arabidopsis genes will be sought using standard cloning techniques based on PCR, genome walking and genomic library screening. The genomic structure and sequence of the genes in biennial beet will be determined and compared with known sequences from other species. Key genes that integrate vernalization and photoperiodic pathways will be isolated in the first instance and as possible; their function verified by complementation of Arabidopsis mutants. Finding these genes in sugar beet is expected to present opportunities for transgenic control of flowering through their direct manipulation. Objective 2: In order to develop strategies for transgenic control of the sugar beet genes, it is important to determine whether they respond to vernalization and photoperiod in the same way as the Arabidopsis homologs. Therefore, the expression profiles of the Beta-specific flowering genes will be predicted by measuring their transcriptional activity in vernalized plants grown under inductive and non-inductive photoperiods.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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