Award details

The role of gibberellin inactivation in the control of plant development

Reference	BBS/E/C/00004568
Principal Investigator / Supervisor	Dr Andrew Phillips
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	132,796
Status	Completed
Type	Institute Project
Start date	05/01/2004
End date	04/01/2007
Duration	36 months

Abstract

The aim of the work is to establish the role of GA 2-oxidases, enzymes involved in the inactivation of GAs, in the regulation of plant development. We will focus on Arabidopsis stem elongation and flowering in short days as models of GA action. A key objective is to test the hypothesis that GA inactivation is not only important in GA homeostasis but is also essential in maintaining tissue-specific action of GAs. There will be a high degree of interaction with existing externally-funded projects on GA 20-oxidases and GA 3-oxidases, and with work in other laboratories, that will provide information on the co-ordination of GA biosynthesis and turnover in the control of developmental processes. We will identify of the patterns of expression of the five GA2ox genes using existing promoter:GUS fusions and RT-PCR. We will also study the fine detail of expression of the genes in the leaves, stem and apex during GA-regulated stem elongation and flowering, using in situ hybridization and immunolocalization, and propose roles for GA 2-oxidases in these processes. This will be correlated with analysis of GA levels in these tissues and with expression of key GA-regulated genes such as LEAFY (in collaboration with SLU Umeå, Sweden). We will test hypotheses about the roles of GA2ox genes in the regulation of flowering and stem elongation through the analysis of knockout mutants and the generation of RNAi lines. The GA status of apical and surrounding tissues will also be manipulated by expression of the GA desaturase gene from Gibberella fujikuroi that will produce GAs resistant to inactivation and thereby test the importance of GA turnover in these processes and in GA homeostasis.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search