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The organogenic activity of compound leaves: interplay between knox proteins and growth hormones

Reference	BBS/E/C/00004543
Principal Investigator / Supervisor	Professor Peter Hedden
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	136,566
Status	Completed
Type	Institute Project
Start date	01/04/2003
End date	09/11/2006
Duration	43 months

Abstract

Compound leaf formation is the result of prolonged organgenic activity within leaf primordia and is thought to be associated with expression of KNOTTED1-like homeobox (KNOX) transcription factors within the primordia. The objective of this project is to investigate the relationship between KNOX expression and metabolism of the plant hormones, gibberellins and cytokinins, in determining leaf dissection in tomato. The investigation will use the following experimental strategies: 1) Determine expression levels of genes involved in GA biosynthesis, catabolism and response in mutants with elevated or reduced KNOX levels and compare the spatial distribution of expression of GA metabolism and KNOX genes and follow temporal changes in their expression in response to altered KNOX content using an inducible 35S::KN1:GR line; 2) Determine changes in growth hormone status resulting from altered KNOX expression (throughout project); 3) Investigate the mechanism whereby altered KNOX content increases cytokinin levels by determining effects on expression of isopentenyltransferase (IPT) genes; 4) Determine genetic interactions between altered leaf dissection mutants and mutants in GA biosynthesis and signalling; 5) Determine the combined effects of altered GA and cytokinin content on leaf morphology. Changes to hormone content will be obtained either by chemical application or by genetic manipulation of biosynthesis or catabolism. Cytokinin content will be increased by overexpressing ipt while overexpression of cytokinin oxidase will reduce cytokinin levels.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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