Award details

The physiological roles of Gibberellin 20-oxidase isozymes in plant development

Reference	BBS/E/C/00004500
Principal Investigator / Supervisor	Professor Peter Hedden
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	4,055
Status	Completed
Type	Institute Project
Start date	01/04/2002
End date	22/07/2005
Duration	40 months

Abstract

The biosynthesis of the gibberellin (GA) hormones is regulated by intrinsic and environmental factors, many of which act on GA 20-oxidase (GA20ox), a rate-limiting enzyme that functions late in the pathway. The Arabidopsisis genome contains five putative GA20ox genes, some which have been shown to be expressed in a distinct tissue-specific manner. The hypothesis that these genes are involved in different developmental processes, such as stem extension, flower development and seed germination, will be tested by isolating lines with knock-out mutations in each GA20ox gene, either by screening populations of T-DNA insertion lines, or by specific gene silencing using RNA interference. Since there is evidence for partial redundancy for the GA20ox genes, multiple gene knock-outs will be produced, either by crossing the single gene mutants or knocking out several genes simultaneously by RNA interference. The phenotypes of the mutants will be correlated with their gene expression patterns, determined using reporter genes and by in situ hybridisation and immunolocalisation.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search