Award details

Transposon tagging of wheat to isolate new promoters and genes involved in stress tolerance

Reference	BBS/E/C/00004301
Principal Investigator / Supervisor	Professor Christine Foyer
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	321,331
Status	Completed
Type	Institute Project
Start date	01/04/2003
End date	31/03/2005
Duration	24 months

Abstract

The aim of this project is to isolate and characterize genes and promoters responsive to stress in wheat. In the first instance, screens will be used to select for oxidative stress-responsive promoters and genes. This will be followed by screening for variations in response to more complex stresses, particularly drought and cold. A population of transposon-tagged wheat lines has been produced, using the Ac/Ds tagging system from maize. Fourteen Ac-lines containing the Ac (Activator) element and 21 Ds-lines containing Ds (Dissociation) elements associated to the reporter gene uidA have been generated and characterized for copy number and segregation patterns by Southern blot. All Ac lines have been characterized by transient GUS assay and show evidence of strong transposase activity. The frequency of transposition of the maize Ac/Ds system in wheat has been estimated in pilot crosses. Nearly 1000 F1 plants were derived from 49 independent crosses and analysed by PCR. 42 percent of the plants containing Ac and Ds elements showed evidence of transposition. Two lines showing a distinctive phenotype were identified and a F1 population produced. These populations are being fully characterised and their tagged promoters/genes cloned. These populations will be used to select for stable tagged lines. The potential application of this project is that many types of promoters can be isolated from these tagged wheat populations (tissue-specific, developmentally-regulated, nutrient-inducible etc.).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search