Award details

A functional genomics approach to the identification of genes determining fungal pathogenesis of cereals

Reference	BBS/E/C/00004187
Principal Investigator / Supervisor	Professor Kim Hammond-Kosack
Co-Investigators / Co-Supervisors	Dr Jason Rudd
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	2,289,418
Status	Completed
Type	Institute Project
Start date	01/04/1999
End date	31/03/2008
Duration	108 months

Abstract

The aim of this project is to characterise and understand the role of genes that condition the pathogenic habit in the economically important cereal fungal pathogen, Mycosphaerella graminicola (anamorph Septoria tritici). This fungus, along with Phaeosphaeria nodorum, is responsible for the Septoria leaf blotch disease of wheat which is estimated to cause annual losses in the UK of c. 330 kt. This is despite the use of fungicides on over 95% of the wheat crops grown in this country. In recent years, M. graminicola has become the more prevalent of the two pathogens, and its increasing importance is reflected in the emphasis placed on M. graminicola in this project. In 2002, field isolates resistant to strobilurin fungicides were identified in several EU countries. Our main objectives are to identify pathogenicity genes and key metabolic and biological processes contributing to infection and colonisation of the host through the use of new emerging genomic technologies. By gaining a better knowledge of the mechanisms underlying pathogenicity and virulence we should be in a better position to identify key events in the disease cycle of these pathogens that determine their ability to incite disease and cause associated crop losses. These targets could then be exploited in the development and discovery of new, novel and environmentally benign control measures for crop improvement to reduce disease incidence. The work involves: 1) bioinformatic analysis of DNA sequence data from cDNA libraries through expressed sequence tags (ESTs) to identify gene function 2) transcription profiling analysis of various biological scenario¿s using an EST fabricated array containing 2,563 distinct unigenes to study overall gene expression during infection and colonisation of the host, 3) targeted gene `knock-out experiments to ascribe gene function.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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