Award details

Molecular cloning, function and structure of gibberellin-biosynthetic enzymes

Reference	BBS/E/C/00004161
Principal Investigator / Supervisor	Professor Peter Hedden
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	1,648,630
Status	Completed
Type	Institute Project
Start date	01/04/1999
End date	31/03/2008
Duration	108 months

Abstract

The gibberellins (GAs) are plant hormones that control development throughout the life cycle of higher plants and mediate responses to environmental signals. The genes that encode the enzymes for the biosynthesis and catabolism of GAs are required in order to determine how GA content is regulated by endogenous and environmental factors and also to investigate the properties of the biosynthetic enzymes. The objective of the project is to isolate and characterise each of the genes involved in GA metabolism. This will be achieved using a variety of techniques and will be aided significantly by the availability the Arabidopsis genome sequence database. The function of the genes will be confirmed by expression in heterologous systems and determining the catalytic activity of the expressed proteins. Recombinant protein will be used to study the reaction mechanisms and structure of the biosynthetic enzymes using isotopically labelled substrates in combination with mass spectrometry and nuclear magnetic resonance spectroscopy, as well as affinity labelling, site-directed mutagenesis and X- ray crystallography.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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