Award details

Development and application of cereal transformation technologies

Reference	BBS/E/C/00004158
Principal Investigator / Supervisor	Professor Huw Jones
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	1,710,171
Status	Completed
Type	Institute Project
Start date	01/04/1999
End date	31/03/2008
Duration	108 months

Abstract

Rothamsted Research occupies a leading position world-wide in the ability routinely to genetically transform commercial varieties of wheat. This project aims to increase our understanding of the processes involved and improve the practical utility of wheat transformation. The project also provides 'service' collaborations for scientists within Rothamsted Research to utilise wheat transgenic technologies in the study of agronomic and end-use quality traits of strategic interest.The genetic transformation of wheat is a technically challenging process confined to a relatively few public-sector laboratories world-wide. During the last decade, we have systematically determined and optimised the variables of DNA delivery and tissue culture that are critical in the wheat transformation process via both biolistics and Agrobacterium. Recent developments have further improved our in-house protocols for transformation via bombardment (eg Pastori et al 2001), and we are refining novel methods for Agrobacterium-mediated DNA transfer to wheat (Amoah et al 2001, Wu et al 2003). As the methods for basic genetic transformation have become increasingly robust, our attention has turned to the mechanisms that control transgene insertion, and also to the isolation, characterisation and utilisation of promoter sequences that deliver transgene expression in particular wheat tissues or at defined stages of development. In this respect we are developing platform technologies for the generation of tagged and knockout wheat lines that will be used to identify genes and promoter sequences and form part of a wider wheat functional genomics initiative within Rothamsted Reseach. There are strong links with other projects, including: 4374, 4149, 4339, 4123, 4038.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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