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Allosteric modulation of biogenic amine G-proteins coupled receptors by endogenously released neuropeptides.

Reference	BBS/E/B/51996693
Principal Investigator / Supervisor	Dr Peter Evans
Co-Investigators / Co-Supervisors
Institution	Babraham Institute
Department	Babraham Institute Department
Funding type	Research
Value (£)	26,300
Status	Completed
Type	Institute Project
Start date	01/12/1997
End date	01/10/1999
Duration	22 months

Abstract

G-protein linked receptors for biogenic amines may be allosterically modulated by endogenous neuropeptides. The project aims to examine this possibility for cloned insect octopamine receptors. Specific neuropeptides expressed in identified locust octopaminergic neurones will be identified using cDNA library subtraction and differential display techniques. The physiological roles of such peptides will be investigated in a model locust neuromuscular preparation with an identified octopaminergic modulatory neurone appropriate to heterologous receptors expression systems. Multidisciplinarity: This project integrates molecular biological cloning approaches with electrophysiological studies of gene expression systems. The likely benefits and outputs of the research would be the identification of novel sites on insect G-protein coupled receptors that could regulate the effects of biogenic amines. Exploitability: Cell lines expression receptors with binding sites for biogenic amines and for allosteric modulatory sites for neuropeptides could be used as the basis for high through-put screens for the identification of novel lead compounds for the development of insect control agents. The project is relevant to the aims of the Agri-Food planning area to improve ecological balance and of the Animal Sciences planning area to improve the biomolecular design of chemicals because it could lead to the development of novel pesticides with a low vertebrate toxicity. For the same reasons there is relevance to ANRE Foresight (biological pest control) and Chemicals Foresight (biochemical technology, environment and safety). It is also relevant to the H&LS Foresight aims to increase understanding in the Molecular Diversity and Molecular Recognition areas of Drug Creation and Delivery.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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