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Role of protective genes in preventing activation and apoptosis in pig endothelial cells
Reference
BBS/E/B/32917794
Principal Investigator / Supervisor
Dr Peter Kilshaw
Co-Investigators /
Co-Supervisors
Institution
Babraham Institute
Department
Babraham Institute Department
Funding type
Research
Value (£)
92,997
Status
Completed
Type
Institute Project
Start date
01/08/1997
End date
01/05/2000
Duration
33 months
Abstract
The significance of Th2 cytokines in xenograft/allograft accommodation is being examined. The effect of I1-4, I1-13 and I1-15 on the transcription of protective genes in cultured endothelial cells is being assessed. A quantitative PCR method has been developed to measure transcription of A20m Bcl-xl and Bcl-2 in cultured pig endothelial cells and in HUVEC. Recent experiments have shown that IL-13 has a striking effect on the transcription of A20 and partially protects endothelial cells against apoptosis induced by TNF- alpha and cyclohexamide. This cytokine also partly inhibits TNFalpha-induced activation of endothelial cells. Experiments using anti-sense oligonucleotides are in progress to assess the relative importance of A20, Bcl-xl and Bcl-2 in protection. In addition, cDNA subtraction is being used to identify novel transcripts induced by IL-13 in cultured endothelial cells that may have a protective role.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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