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Functional imaging of receptors in living cells by 2-photon uncaging of signaling molecules

Reference	BBS/E/B/0000P068
Principal Investigator / Supervisor	Dr Martin Bootman
Co-Investigators / Co-Supervisors
Institution	Babraham Institute
Department	Babraham Institute Department
Funding type	Research
Value (£)	95,118
Status	Completed
Type	Institute Project
Start date	01/05/2003
End date	30/04/2005
Duration	24 months

Abstract

The binding of signalling molecules to their receptors is fundamental process for many cellular responses. The goal of this project is to develop and apply new caged neurotransmitters and second messengers that will permit 3-dimensionally defined photorelease of these signalling molecules at synapses and intracellular sites via 2-photon photolysis (2PP). Two-photon excitation has proved extremely valuable for cellular imaging and, in principle, should allow 2PP of caged effectors. Unfortunately, currently available caged compounds are relatively insensitive to 2PP. This project will develop and test new chromophores that are effective for 2PP (In collaboration with Professor Graham Ellis-Davies, Philadelphia). The unprecedented spatial and temporal control of effector molecule application provided by these compounds will be exploited to examine the physiological properties of neurotransmitter receptors at single synapses (in collaboration with Professor Haruo Kasai, Okazaki) and of second messengers in HeLa cells and cardiac myocytes.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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