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Specification of promordial germ cells from pluripotent cells.

Reference	BBS/B/14590
Principal Investigator / Supervisor	Professor Azim Surani
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Gurdon Institute
Funding type	Research
Value (£)	219,686
Status	Completed
Type	Research Grant
Start date	01/10/2004
End date	30/09/2007
Duration	36 months

Abstract

A systematic approach will be developed for the derivation of primordial germ cells (PGCs) from pluripotent embryonic stem cells (ES) and embryonic germ cells (EG). ES/EG cells with fragilis-GFP and stella-GFP reporters will be used to assess their progression towards germ cell competence and specification, respectively. ES/EG cells with nanog-YFP and stella-CFP, will also be used to monitor the conversion pluripotent stem cells into germ cells. Both extracellular signalling molecules and intrinsic regulatory factors will be tested to drive the ES/EG cells towards PGC fate. Successful protocols will be tested on human ES cells. In vitro derived PGCs will be fully characterised, and assessed for the unique epigenetic reprogramming events. From these essential first steps, we shall explore the eventual derivation of mouse and human oocytes from ES/EG cells.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Stem Cell Science and Engineering Initiative (SCE) [2003-2005]
Funding Scheme	X – not Funded via a specific Funding Scheme

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