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A proteomic characterisation of embryonal stem cell development

Reference	BBS/B/14507
Principal Investigator / Supervisor	Professor Anthony David Whetton
Co-Investigators / Co-Supervisors	Dr Raj Chopra, Professor Simon James Gaskell, Professor Simon Hubbard, Dr Valerie Kouskoff, Professor Georges Lacaud
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	472,448
Status	Completed
Type	Research Grant
Start date	12/07/2004
End date	11/08/2008
Duration	49 months

Abstract

The objective of this multidisciplinary project is to apply proteomics expertise to embryonal stem (ES) cell development along the mesoderm/hemangioblast path. Changes in transcription do not directly correlate to changes in protein expression. We will define changes in embryonal stem cell proteome and phosphoprotein complement as they develop, and using microarray data from a parallel project, compare this to transcriptome changes. ES cell commitment to mesoderm will be monitored using brachyury expression and formation of hemangioblasts with VEGF receptor 2 expression. The role of the key protein Runx1 will also be assessed. Further development to haematopoietic progenitor cells can be accomplished with growth factor addition. Using sorted cell populations we can therefore assess the proteome and analyse phosphorylation systematically as cells undergo development.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	Stem Cells
Research Priority	X – Research Priority information not available
Research Initiative	Stem Cell Science and Engineering Initiative (SCE) [2003-2005]
Funding Scheme	X – not Funded via a specific Funding Scheme

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