Award details

Large scale functional genomics in chicken embryos

Reference	BBS/B/13578
Principal Investigator / Supervisor	Professor Stuart Wilson
Co-Investigators / Co-Supervisors	Professor Marysia Placzek
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	252,062
Status	Completed
Type	Research Grant
Start date	01/01/2005
End date	31/12/2006
Duration	24 months

Abstract

We have devised a method to perform RNA interference (RNAi) in chicken embryos using PCR products. Using in silico subtraction we have identified a set of around 1000 chicken genes which are expressed in embryonic limbs and neural tube. We will use RNAi to knockdown expression of this genes set in the limb and neural tube and identify genes that are required for limb bud outgrowth and patterning and neural tube differentiation. We will also test the function of a collection of chicken microRNAs which we have identified using misexpression studies. This project will generate a resource of chicken RNAi PCR products and vectors which will be made available to the wider scientific community via ARK Genomics. (Joint with BBS/B/13594)

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BBS/B/13594 Large scale functional genomics in chicken embryos

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