Award details

Structural mechanistic and genetic studies of a fluorination enzyme and fluorometabolite pathway in Streptomyces cattleya

Reference	BBS/B/13357
Principal Investigator / Supervisor	Dr Jonathan Spencer
Co-Investigators / Co-Supervisors	Professor James Naismith, Professor David O'Hagan
Institution	University of Cambridge
Department	Chemistry
Funding type	Research
Value (£)	188,065
Status	Completed
Type	Research Grant
Start date	24/07/2004
End date	23/03/2008
Duration	44 months

Abstract

This collaborative programme between St Andrews and Cambridge will explore fluorometabolite production in Streptomyces cattleya at the genetic, enzymatic and structural level. The programme develops from the recent identification of the first native fluorination enzyme, its cloning and solution of an X-ray structure of the enzyme. In particular SDM and crystallisation studies will be carried out to probe the mechanism of biological fluorination. The programme will extend to full sequencing of the biosynthesis gene cluster and over-expression of the relevant enzymes for structural studies. Knock out mutants and cloning of the entire fluorometabolite pathway will be explored such that new fluorinated products can be prepared and enzymatic fluorination can be manipulated into other micro-organisms with the prospects of developing the first biotechnological methods for organo-fluorine compounds. (Joint with BBS/B/04579).

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BBS/B/04579 Structural mechanistic and genetic studies of a fluorination enzyme and fluorometabolite pathway in streptomyces cattleya

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