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Use of mice transgenically expressing RFP-tagged TAPP PH domain to study spatio-temporal signalling in T lymphocytes

Reference	BBS/B/13330
Principal Investigator / Supervisor	Professor Stephen Ward
Co-Investigators / Co-Supervisors	Dr Kim Dora, Professor Christopher Garland
Institution	University of Bath
Department	Pharmacy and Pharmacology
Funding type	Research
Value (£)	198,451
Status	Completed
Type	Research Grant
Start date	01/05/2004
End date	30/04/2007
Duration	36 months

Abstract

PI3K is an important signalling molecule involved in several aspects of T cell biology. It is presently unclear whether the PI3K lipid product. PI(3,4)P2, accumulates at distinct cellular locations and whether it can be formed independently of PI(3,4,5)P3 metabolism. The principle aim of this study therefore is to visualise the spatio-temporal regulation of PI(3,4)P2 in T lymphocytes undergoing two distinct types of polarised response. We will create and use mice transgenically expressing a fluorescently-tagged TAPP PH domain with specificity for PI(3,4)P2, in order to visualise this phosphoinositide lipid in T lymphocytes undergoing (i) directional migration towards chemokines and (ii) stimulation of the T cell antigen receptors.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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