Award details

Analysis and exploitation of genes and proteins involved in high frequency lactococcal conjugation

Reference	BBS/B/12318
Principal Investigator / Supervisor	Dr Claire Shearman
Co-Investigators / Co-Supervisors	Professor Michael Gasson, Dr Regis Stentz
Institution	Quadram Institute Bioscience
Department	Food Safety Science Division
Funding type	Research
Value (£)	201,485
Status	Completed
Type	Research Grant
Start date	01/08/2004
End date	31/07/2007
Duration	36 months

Abstract

A highly efficient conjugation system in the industrially important species Lactococcus lactis will be further characterised. Conjugal gene transfer is important in bacterial evolution and for the safety evaluation of GM technology. It is a valuable genetic tool for non-recombinant strain of construction, especially in food-use microorganisms. This conjugation system involves a chromosomal sex factor with unique properties, including the ability to mobilise the bacterial chromosome. We will focus on genes involved in mating pair formation and DNA transfer, concentrating on cluA and four traG homologues. The global expression of sex factor genes will be analysed, including the use of DNA microarrays, in order to identify their regulation.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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