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Does isoprene emission protect plants against thermal stress and is this trait transferable into plant species that do not produce isoprene?

ReferenceBBS/B/12172
Principal Investigator / Supervisor Professor Charles Hewitt
Co-Investigators /
Co-Supervisors
Institution Lancaster University
DepartmentEnvironmental Science
Funding typeResearch
Value (£) 217,592
StatusCompleted
TypeResearch Grant
Start date 01/03/2004
End date 28/02/2007
Duration36 months

Abstract

The capacity to make isoprene (2-methyl-1,3-butadiene) has been hypothesised to confer thermotolerance on plants. Isoprene synthesis is catalysed by chloroplast located isoprene synthase (IS). Initially, we will test the this hypothesis using hybrid poplar inhibited for IS activity. More conclusively, we will test if over-expression of IS cDNAs from poplar in transgenic tobacco (a non-emitter of isoprene) allows the latter to emit this compound and if so, are such tobaccos more thermotolerant than their wild-type siblings. This will provide conclusive evidence for the thermotolerance hypothesis and will open the possibility of enhancing heat stress tolerance in crops by genetic manipulation. We shall couple such studies to a characterisation of IS isoforms and genes in poplar and to determine if post-translational activation of the enzyme occurs under heat stress. (Joint with BBS/B12237).

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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