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Award details
The creation of mouse models to study the role of the Wilms' Tumour suppressor gene (WT1) in muscle development
Reference
BBS/B/11699
Principal Investigator / Supervisor
Dr CG Miles
Co-Investigators /
Co-Supervisors
Institution
Newcastle University
Department
Institute of Human Genetics
Funding type
Research
Value (£)
218,847
Status
Completed
Type
Research Grant
Start date
01/07/2004
End date
31/08/2007
Duration
38 months
Abstract
Loss of WT1 function promotes myogenesis in Wilms tumour and WT1 expression blocks myoblast differentiation. This proposal aims to use WT1 regulatory elements, conserved from fish to man, to create transgenic mice for the study of WT1 in muscle development. Novel Cre expressing transgenic mice will be generated to facilitate tissue (muscle) specific WT1 gene knockout in vivo for direct assessment of the function of WT1 in muscle progenitor cells. The significance of WT1 down-regulation during muscle development will be assessed by precise targeting of ectopic WT1 expression in vivo, resulting in persistent WT1 expression throughout muscle development. The generality of any findings will be assessed by comparison with the chick.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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