Award details

Tackling the problem of 16S rRNA chimeras through analysis of sequence regularity - a software solution

Reference	BBS/B/11494
Principal Investigator / Supervisor	Professor Andrew Weightman
Co-Investigators / Co-Supervisors	Dr Kevin Ashelford, Professor Nadia Chuzhanova, Professor John Fry, Professor Antonia Jones
Institution	Cardiff University
Department	School of Biosciences
Funding type	Research
Value (£)	172,662
Status	Completed
Type	Research Grant
Start date	01/03/2004
End date	28/02/2007
Duration	36 months

Abstract

Systematic bacteriology relies on 16S rRNA data. Errors while collecting this date compromise public databases by giving a false impression of diversity and phylogeny. A frequent error is chimera formation resulting from the concatenation of phylogenetically distinct gene fragments during PCR. Current ways of detecting chimeras are unreliable. We will therefore develop a new algorithm using complexity analysis. Our method will exploit secondary structure information, complementing existing primary structure approaches. We will write a user-friendly, platform-independent software tool that will combine our algorithm with current methods to create a robust, comprehensive chimera solution of value to researchers and database managers alike.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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