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The mechanism of target mRNA degradation in post-transcriptional gene silencing in plants

Reference	BBS/B/10846
Principal Investigator / Supervisor	Professor Donald Grierson
Co-Investigators / Co-Supervisors	Dr Yuanhuai Han
Institution	University of Nottingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	237,832
Status	Completed
Type	Research Grant
Start date	01/04/2004
End date	31/03/2007
Duration	36 months

Abstract

We aim to understand the requirements for and mechanism of mRNA targeting and degradation in post-transcriptional gene silencing in plants. We will use a wheat germ in vitro system to determine the specific template preference of RNA-dependent RBA polymerase for different versions of the same mRNA including transgene and endogenous mRNAs and synthetic RNAs with and without inverted repeats (IRs). We showed previously that siRNAs from a truncated polygalacturonase (PG) transgene were linked with the cleavage of endogenous PG mRNA into two fragments, and proposed a silencing model. We will make new truncated constructs with and without IRs to test our model in tomato and address the requirements for mRNA targeting and degradation.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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