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Cell biology of phage abortive infection: proteomics and functional genomics of plasmid-encoded altruistic apoptosis

Reference	BBS/B/10722
Principal Investigator / Supervisor	Professor George Salmond
Co-Investigators / Co-Supervisors	Professor Kathryn Lilley
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	240,153
Status	Completed
Type	Research Grant
Start date	01/06/2004
End date	31/12/2007
Duration	43 months

Abstract

A cryptic plasmid in the phytopathogen, Erwinia, encodes a phage abortive infection (Abi) system active against multiple phages. We will use DiGE proteomic analysis and genetics to identify the molecular basis of the abortive infection system. We will determine the location of the Abi protein, assess the phage inducibility of the cognate gene and the impact of artificial overproduction. We will monitor Abi protein synthesis in the lytic cycle of an Abi-sensitive phage. The phage genome will be sequenced and escape mutants characterised proteomically and genetically. Proteins that interact with the Abi system will be identified and investigated by genetical and biochemical methods. The distribution of the Abi system will be assessed.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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