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NMR studies of membrane associated proteins
Reference
BBS/B/10714
Principal Investigator / Supervisor
Professor Michael Overduin
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
Cancer Sciences
Funding type
Research
Value (£)
450,625
Status
Completed
Type
Research Grant
Start date
01/10/2004
End date
30/09/2007
Duration
36 months
Abstract
The overall goal is to understand how membrane-binding domains insert into bilayers and bind phospholipids to target proteins to subcellular organelles. Targets include the PX domain (aim 1) and the DIX domain (aim 2). Structures of the phospholipid-bound and micelle-inserted states will be determined by NMR spectroscopy. The depth and angles of bilayer insertion will be measured using paramagnetic spin labels. The binding specificities and affinities for lipids will be measured. Regulatory mechanisms including protein interactions will be investigated. Residues that contact ligands will be mutated to test effects on subcellular localisation and in vivo interactions of full-length proteins.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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