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Atomic force microscopy of restriction-modification enzymes - elucidating the mechanisms of molecular machines

Reference	BBS/B/1065X
Principal Investigator / Supervisor	Dr Robert Henderson
Co-Investigators / Co-Supervisors	Dr David Dryden, Professor John Edwardson
Institution	University of Cambridge
Department	Pharmacology
Funding type	Research
Value (£)	202,185
Status	Completed
Type	Research Grant
Start date	01/06/2004
End date	31/10/2007
Duration	41 months

Abstract

Many restriction enzymes function as complex molecular machines, making them ideal model systems for study of DNA looping (caused by the enzyme binding to multiple sites on a DNA molecule) and DNA translocation, whereby the enzyme can move large lengths of DNA. In earlier work we used atomic force microscopy to study the mechanism of the Type I restriction enzyme EcoKI. Here we will extend our understanding of EcoKI by studying factors involved in control of the rate of translocation and processing of DNA; Examine loop formation more closely by taking AFM snapshots of the process, and by using combed DNA; Investigate the mechanism of action of EcoKI on condensed DNA and finally; Extend our studies to appropriate Type II and III restriction enzymes functionally related to EcoKI, examining the topology of dimerisation and processivity of these enzymes.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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