Award details

Understanding and exploiting the mechanism by which the multienzyme cellulosme complex is assembled in Clostridium thermocellum

Reference	BBS/B/10358
Principal Investigator / Supervisor	Professor Harry Gilbert
Co-Investigators / Co-Supervisors	Professor Gideon Davies, Professor Michael Williamson
Institution	Newcastle University
Department	Inst for Cell and Molecular Biosciences
Funding type	Research
Value (£)	307,352
Status	Completed
Type	Research Grant
Start date	01/10/2004
End date	30/09/2007
Duration	36 months

Abstract

Multienzyme plant cell wall degrading complexes, synthesised by anaerobic bacteria such as Clostridium thermocellum, are assembled by the interaction of two protein domains named cohesions and dockerins. In this project we will exploit the recent three-dimensional structure of the C. thermocellum dockerin-cohesin complex to understand the structural basis for the specificity of these protein interactions. The data will then be exploited in engineering novel specificities into cohesin-dockerin recognition, which will facilitate the construction of designer macromolecular assemblies to orchestrate any enzyme-catalysed reactions which might benefit from component proximity. The cohesion-dockerin structure will thus form a blueprint for tailored multi-component catalytic machines across a range of biological processes.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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