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Role of plant amino acid transporters in determining the performance of phloem-feeding aphids: functional genomics in Arabidopsis

Reference	BBS/B/09872
Principal Investigator / Supervisor	Dr Jeremy Pritchard
Co-Investigators / Co-Supervisors	Professor Jeffrey Bale, Professor David Barrett, Professor Henry John Newbury
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	201,978
Status	Completed
Type	Research Grant
Start date	15/10/2004
End date	14/10/2007
Duration	36 months

Abstract

This study will use genetic resources in the model plant Arabidopsis to dissect the complex relationship between diet and performance of the phloem-feeding aphid Myzus persicae. Phloem amino acid concentrations are central to aphid performance, but the effect of a sub-optimal diet is difficult to predict due to a range of compensatory responses that the aphid can employ. We will modify phloem sap without knockout mutations of defined plant amino acid transporters. Phloem composition will be monitored using extraction (stylectomy) and analysis (capillary electrophoresis, CE). The effect of modified diets on aphid performance will be measured and compensatory responses characterised by electrical penetration graphs (changes in feeding time in phloem), honeydew clocks (altered flux through the insect), and CE (variation in aphid uptake of amino acids).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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