Award details

Targeted TILLinG to rapidly identify conserved and novel membrane trafficking loci in Arabidopsis

Reference	BBS/B/09562
Principal Investigator / Supervisor	Professor Ian Moore
Co-Investigators / Co-Supervisors	Professor Miltos Tsiantis
Institution	University of Oxford
Department	Plant Sciences
Funding type	Research
Value (£)	264,796
Status	Completed
Type	Research Grant
Start date	01/11/2004
End date	31/10/2007
Duration	36 months

Abstract

In plant cell biology, forward genetic screens followed by costly positional cloning exercises run the risk of simply rediscovering loci with well characterised orthologues. We will use TILLinG to rapidly screen a population of 50-100 putative trafficking mutants for mutations in the 400 or so Arabidopsis genes that are related to be known trafficking genes in other organisms. In this way, within 3 years we expect to have identified the mutant phenotypes of numerous mutable loci in the Arabidopsis membrane trafficking pathways and to have identified a second set of mutants whose essential role in membrane traffic cannot be identified simply on the basis of sequence. Genomic resources will allow rapid validation of mutant phenotypes assigned by TILLinG while positional cloning can be justified in future for mutants that cannot be assigned a candidate gene.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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