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Proteomic definition of dendritic cell phenotypes matured in response to helminth antigens

Reference	BBS/B/08531
Principal Investigator / Supervisor	Dr Adrian Mountford
Co-Investigators / Co-Supervisors	Mr Stephen Jenkins
Institution	University of York
Department	Biology
Funding type	Research
Value (£)	220,648
Status	Completed
Type	Research Grant
Start date	01/08/2004
End date	30/04/2008
Duration	45 months

Abstract

Gel-based and gel-free proteomics will be used to define the maturation phenotype of dendritic cells (DC) stimulated with various helminth and microbial products, known to induce Th1, or Th2 immune responses. Our goal is to identify signature proteins with putative immune function in differentially matured DCs. Changes in DC proteome profiles will be analysed at different maturation times, doses of stimulant, and in the presence of known polarising agents (anti-CD40). We will also examine the effect of using alternative helminth products as maturation stimuli, and use DCs of skin origin. Differential expression of signature proteins will be confirmed using western blotting, flow cytometry and/or functional assays where feasible.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	Animal Health, Immunology
Research Priority	X – Research Priority information not available
Research Initiative	Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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