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Identification and characterisation of proteins that interact with the Drosophila sex determination transcription factor Fruitless

Reference	BBS/B/0837X
Principal Investigator / Supervisor	Professor Stephen Goodwin
Co-Investigators / Co-Supervisors	Dr Megan Neville
Institution	University of Glasgow
Department	Molecular Genetics
Funding type	Research
Value (£)	285,013
Status	Completed
Type	Research Grant
Start date	01/06/2004
End date	31/03/2008
Duration	46 months

Abstract

To improve our understanding of the biological function of the Drosophila sex determinant transcription factor Fruitless (FRU) we will use complementary approached aimed at identifying factors that interact with FRUM proteins. We propose to use a yeast two-hybrid screen and a TAP-tag-purification strategy coupled with mass spectrophotometry to identify fruitless-interacting proteins (FIPs) directly from Drosophila. Purification coupled with mass spectrophotometry is capable of identifying protein complexes while the two-hybrid approach is able to map interactions within these complexes. It is notable that both of these approaches are greatly aided by the recent completion of the Drosophila genome. The relevance of interactions identified using these techniques will subsequently be verified by genetic, molecular and biochemical means.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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