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Identification and role in virulence of Actinobacillus pleuropneumoniae sigmaE-regulated genes

Reference	BBS/B/08132
Principal Investigator / Supervisor	Professor Paul Langford
Co-Investigators / Co-Supervisors	Dr Janine Bosse, Professor John Kroll, Professor Andrew Rycroft
Institution	Imperial College London
Department	Dept of Medicine
Funding type	Research
Value (£)	244,665
Status	Completed
Type	Research Grant
Start date	01/03/2004
End date	30/04/2007
Duration	38 months

Abstract

RpoE (omega E) is sigma factor that has a major role in regulating responses of bacteria to extracytoplasmic stresses, such as heat shock and oxidative stress. We have identified a homologue of omega E in the economically important swine pathogen Actinobacillus pleuropneumoniae (APL). In a signature tagged mutagenesis screen in pigs, we have identified mutants with interruptions in rpoE and rseA (encoding the negative regulator of omega E) that are attenuated for virulence. We aim to 1) determine the conditions which induce transcription of omega E-regulated genes 2) identify members of the omega E regulon and 3) further assess the contribution of omega E, and selected members of the omega E regulon, to the pathogenesis of APL infection in its natural host, the pig. Our results will increase our understanding of the molecular basis of APL infection and may generate novel live vaccine candidates.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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