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Award details
Identification and characterisation of mitotic phospho-proteins in Drosophila
Reference
BBS/B/08019
Principal Investigator / Supervisor
Professor James Wakefield
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Zoology
Funding type
Research
Value (£)
233,300
Status
Completed
Type
Research Grant
Start date
01/10/2004
End date
31/10/2007
Duration
37 months
Abstract
This proposal aims to use a proteomic approach to identify proteins that have a crucial role during cell division in Drosophila. We possess an antibody that recognises mitotic phospho-epitopes conserved between Drosophila and human cells. We will subject Drosophila embryo extracts to a series of chromatography-based purification steps followed by mass spectrometry to identify mitotically regulated proteins. We will focus on the Drosophila genes identified that possess mammalian homologues. Their function will be addressed through live imaging of GFP tagged proteins in Drosophila embryos and through mutational analysis. These studies will aid in our understanding of the fundamental biological process of cell division.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme
X – not Funded via a specific Funding Scheme
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