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Determining the role of trehalose synthesis and metabolism during plant infection
Reference
BBS/B/08000
Principal Investigator / Supervisor
Professor Nicholas Talbot
Co-Investigators /
Co-Supervisors
Institution
University of Exeter
Department
Biosciences
Funding type
Research
Value (£)
235,282
Status
Completed
Type
Research Grant
Start date
01/08/2004
End date
31/07/2007
Duration
36 months
Abstract
Trehalose metabolism plays an essential role in virulence of the rice blast fungus Magnaporthe grisea. This project will test the hypothesis that trehalose-6-phosphate (T6P) synthesis is necessary for regulation of sugar metabolism during appressorium-mediated plant infection. Extragenic suppressors of the non-pathogenic delta- tps1 mutant will be characterised, and mutants lacking T6P phosphatase (TPS2) and hexokinase (HXK1) will be generated in wild type and delta- tps1backgrounds. Transcriptional profiling and parallel biochemical analysis of sugars and phosphorylated intermediates will be performed to investigate the role of TPS1. M. grisea mutants lacking neutral trehalase, which do not colonise leaf tissue effectively, will be also characterised by reporter gene analysis and enzyme assay. The regulation of NT by the cAMP response pathway will be investigated.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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