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Elucidating mechanism and specificity in pectin methylesterase and pectate lyase 9A

Reference	BBS/B/07896
Principal Investigator / Supervisor	Professor Richard Pickersgill
Co-Investigators / Co-Supervisors	Professor K Brocklehurst
Institution	Queen Mary University of London
Department	Sch of Biological and Chemical Sciences
Funding type	Research
Value (£)	204,721
Status	Completed
Type	Research Grant
Start date	01/10/2004
End date	31/10/2007
Duration	37 months

Abstract

We shall elucidate the mechanism and specificity of pectin methylesterase and pectate lyase 9A by determining the three-dimensional structures of numerous, inactive, mutants co-crystallized with substrate and by mechanism-targeted kinetic studies of native and mutant proteins. Alternative mechanisms suggested by the structural studies will be distinguished by the presence or absence of kinetic isotope effects and the number of in flight protons. Using this combined approach we shall identify the key enzyme/substrate interactions involved along the reaction coordinate and elucidate mechanism. This work will reveal the key interactions responsible for rate enhancement by esterases and anti-beta-eliminating lyases.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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