Award details

Amino-acid catabolism in Campylobacter jejuni: biochemical analysis and identification of pathways essential for colonisation

Reference	BBS/B/07667
Principal Investigator / Supervisor	Professor Dave Kelly
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	214,039
Status	Completed
Type	Research Grant
Start date	01/10/2004
End date	30/09/2007
Duration	36 months

Abstract

Campylobacter jejuni cannot catabolise sugars and must rely on other carbon sources for growth in vivo. We recently obtained evidence that amino-acid catabolism is crucial, as mutants unable to convert L-serine to pyruvate and ammonia via an oxygen-labile serine dehydratase are unable to colonise the avian gut. In this project we will (i) undertake a comprehensive analysis of catabolic pathways for asparate, asparagine, glutamate, glutamine and proline in wild-type and mutants cells in vitro, using a combination of transport assays, enzyme characterisation and NMR-analysis (ii) determine which of these pathways are important in vivo, by chicken colonisation studies with defined mutants. The results could underpin novel intervention strategies aimed at limiting the growth of C. jejuni in poultry by preventing catabolism of specific amino-acids. (Joint with BBS/07772)

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BBS/B/07772 Amino-acid catabolism in Campylobacter jejuni: biochemical analysis and identification of pathways essential for colonisation

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