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The molecular basis of anthelmintic resistance in parasitic nematodes and the development of rapid detection systems

ReferenceBBS/B/07594
Principal Investigator / Supervisor Dr Adrian John Wolstenholme
Co-Investigators /
Co-Supervisors
Institution University of Bath
DepartmentBiology and Biochemistry
Funding typeResearch
Value (£) 205,098
StatusCompleted
TypeResearch Grant
Start date 01/07/2004
End date 30/06/2007
Duration36 months

Abstract

Parasitic nematodes are a major threat to UK livestock and resistance to currently used treatments is growing. We will study the molecular basis of anthelmintic resistance in T. circumcincta and H. contortus to produce quantitative PCR assays for the detection of resistance alleles. For benzimidazole resistance, the assay will be based on identified mutations in beta-tubulin genes. For levamisole resistance, where no candidate mutation has been proposed, we look for changes in the nicotinic receptor target site and will also take a proteomics approach to identify genes whose expression changes in resistance worms or following drug treatment. For invermectin resistance, we will use functional genomics techniques to test candidate resistance genes.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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