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Characterisation of eIF2B mutations and their effects on physiology and gene expression in S. cerevisiae and C. albicans

Reference	BBS/B/07500
Principal Investigator / Supervisor	Professor Mark Peter Ashe
Co-Investigators / Co-Supervisors	Professor Aline Miller
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	234,638
Status	Completed
Type	Research Grant
Start date	30/09/2004
End date	29/09/2007
Duration	36 months

Abstract

eIF2B catalyses one of the key regulated steps of protein synthesis, which is the recycling of the translation initiation factor, eIF2-GDP, into the active GTP bound form. Allelic differences in the gamma subunit of eIF2B (coded by the GCD1 gene) lead to translational inhibition in response to fusel alcohols. In preliminary work, we have found that strains with different GCD1 mutant alleles show variations in a range of physiological properties related to colony growth, cell morphology and flocculation. We aim to characterise these phenotypes and the downstream genes that are regulated as a consequence of the allelic variation. We will then analyse the role of translational control in the observed phenotypic variation.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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