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Improving cross-kingdom techniques for siRNA detection and their specific application to x-chromosome inactivation

Reference	BBS/B/07306
Principal Investigator / Supervisor	Dr Andrew Hamilton
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	Institute of Cancer Studies
Funding type	Research
Value (£)	184,735
Status	Completed
Type	Research Grant
Start date	01/06/2004
End date	31/05/2007
Duration	36 months

Abstract

Key to understanding the natural role and regulation of RNA interference in epigenetics will be the ability to reliably and sensitively detect short RNA duplexes known as siRNA which are central components of the RNAi molecular machinery. Current methods are inadequate for the detection of low abundance siRNA. I have identified the limitations of these procedures and propose three improvements: 1, better siRNA retention on hybridisation supports; 2, a novel siRNA enrichment procedure; 3, a novel amplification based method. Cross kingdom applicability of each method will be tested using natural plant and mammalian siRNA. Novel siRNA from the mouse Xist locus will be analysed in detail using the most successful method developed.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Integrated Epigenetics (EPI) [2003]
Funding Scheme	X – not Funded via a specific Funding Scheme

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