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Biosynthesis of the polyketide antibiotic mupirocin by Pseudomonas fluorescens

Reference	BBS/B/07071
Principal Investigator / Supervisor	Professor Christopher Thomas
Co-Investigators / Co-Supervisors	Dr John Crosby, Dr Joanne Hothersall, Professor Thomas Simpson
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	355,624
Status	Completed
Type	Research Grant
Start date	14/05/2004
End date	13/05/2007
Duration	36 months

Abstract

Mupirocin, a polyketide antibiotic used against Methicillin Resistant Staphylococcus aureus, is composed of monic acid and 9-hydroxynonanoic acid and works by inhibiting isoleucyl-tRNA synthetase. Synthesis of monic acid requires multifunctional (Type I) polypeptides, but about half of the biosynthetic cluster consists of essential unifunctional polypeptides. The genes provide great potential for derivation of novel bioactive structures, but rational manipulation depends on greater understanding of the pathway. General ways to study biosynthetic intermediates attached to acyl carriers proteins will be developed. Modules for early steps of the biosynthetic pathway will be dissected to find routes to structural diversity. Related pathways in other organisms will be identified. The location of the biosynthetic complex in the bacterial cell will be determined.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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