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In vivo analysis of higher order chromatin structure in an imprinting cluster

Reference	BBS/B/06040
Principal Investigator / Supervisor	Professor Wolf Reik
Co-Investigators / Co-Supervisors	Dr Peter Fraser
Institution	Babraham Institute
Department	Developmental Genetics and Imprinting
Funding type	Research
Value (£)	207,517
Status	Completed
Type	Research Grant
Start date	01/04/2004
End date	31/03/2007
Duration	36 months

Abstract

Imprinted genes occur in clusters and share control sequences such as enhancers, silencers, and insulators. Various models exist of how these remote sequences can interact, and most invoke higher order chromatin structures (looping). We have developed a transgenic system in which physical contact between remote seqences can be probed by Gal4 targeting and chromatin crosslinking. This system and the recently developed RNA-TRAP method will be employed to examine looping in the Igf2-H19 imprinted region, particularly between enhancers and the genes, and between insulators and silencers. The Gal4 in vivo targeting system will also be used to isolate novel proteins bound to the imprinting centre (IC) and to target known factors such as CTCF and methyltransferases to the IC in vivo to manipulate its function. The work will provide significant insights and applications in epigenetic diseases, cancer, and epigenetic reprogramming.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Integrated Epigenetics (EPI) [2003]
Funding Scheme	X – not Funded via a specific Funding Scheme

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