Award details

Topo II and the epigenetic centromere: studies in higher and lower eukaryotes

Reference	BBS/B/04994
Principal Investigator / Supervisor	Dr Christine Farr
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Genetics
Funding type	Research
Value (£)	203,337
Status	Completed
Type	Research Grant
Start date	19/09/2004
End date	18/09/2007
Duration	36 months

Abstract

Recent studies on human centromeres have revealed a major site of topo II cleavage. Experiments will be carried out to determine the relationship on this activity with the centromere-specific CENP-A chromatin and to establish whether topo II is involved in imprinting the site of kinetochore assembly. Like many eukaryotes vertebrates have two forms of this enzyme, alpha and beta, encoded by independent genes. RNAi will be used to determine the relative contribution of two isoforms to the centromeric cleavage footprint. Finally we will examine whether topo II plays a role at the centromete in two other eukaryotes for which molecularly well defined minichromosomes/centromeres are available - Drosophila and Schizosaccharomyces pombe.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Integrated Epigenetics (EPI) [2003]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search