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Chromatin packaging in mammalian spermatozoa in relation to differential
Reference
BBS/B/04900
Principal Investigator / Supervisor
Dr David Miller
Co-Investigators /
Co-Supervisors
Professor Adam Balen
,
Dr Martin Brinkworth
,
Dr D Iles
Institution
University of Leeds
Department
School of Medicine
Funding type
Research
Value (£)
167,178
Status
Completed
Type
Research Grant
Start date
01/10/2004
End date
31/03/2008
Duration
42 months
Abstract
We aim to test the hypothesis that the nucleosomal domains of mammalian sperm contain gene sequences that are more susceptible to DNA damage. To this end, the differential loading of histone and protamine-associated gene sequences will be examined using oligonucleotide and genomic DNA based micro-arrays. The relative sensitivity of differentially packaged gene sequences to DNA damage will be investigated by exposing murine, bovine, porcine and human sperm to DNA damage-inducing conditions and examining the migration of specific gene sequences by a specialised comet-FISH assay.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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