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Genetic and structural characterisation of novel N-linked general glycosylation systems in eubacteria
Reference
BBS/B/04862
Principal Investigator / Supervisor
Professor Brendan Wren
Co-Investigators /
Co-Supervisors
Institution
London Sch of Hygiene and Trop Medicine
Department
Infectious and Tropical Diseases
Funding type
Research
Value (£)
201,481
Status
Completed
Type
Research Grant
Start date
01/06/2004
End date
31/05/2007
Duration
36 months
Abstract
We recently characterised a novel N-linked general glycosylation system (gss) from the epsilon-proteobacterium Campylobacter jejuni, that has several eukaryotic features (Wacker et al., Science, vol 298, p 1790). We have now identified a second such pathway, with five additional genes, in the related epsilon-proteobacterium Wolinella succinogenes (Wsggs). We cloned the entire pathway into E. coli to produce recombinant glycoprotein. In this research programme, we aim to characterise the complex Wsggs by mutagenesis and structural analysis, and to identify and characterise further ggs pathways in epsilon-proteobacteria. Characterising this new family of glycosylation systems will facilitate glycoengineering, with broad research and industrial applications.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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